Route 35S provides service between the Oxon Hill Park and Ride Lot and the Fort Washington Manor Senior Residences. Key stops include the Gaylord Hotel, Tanger Outlets, and the Old Fort Village Shopping Center.  Riders can access Capital Bikeshare at National Harbor, Tanger Outlets, and the Oxon Hill Park and Ride Lot. Route 35S also provides a connection to the MGM Casino. Route 35S connects with Route 35 at the Oxon Hill Park and Ride Lot.

Route 35S currently operates 75 minute frequency during the AM peak period from 9:00am to 12:35pm. In the PM peak period, frequency is 75 minutes from 12:50pm to 4:32 pm.