Route 35 provides service from the Southern Avenue Metrorail Station to the National Harbor community and Padgett’s Corner in Camp Springs. Key stops include Southern Regional Technology and Recreation Center, United Medical Center (DC), Rivertown Commons Shopping Center, MGM Casino, and Oxon Hill Park and Ride Lot. Capital Bikeshare can be accessed at the Southern Avenue Metrorail Station, United Medical Center, and Oxon Hill Park and Ride Lot. Route 35 connects with Routes 30, 32, and 33 in Camp Springs on Old Branch Avenue at Allentown Way and with Metrobus routes NH1, and NH2 at the Oxon Hill Park and Ride Lot.

Route 35 currently operates with 40 minute frequency. Service span is 5:55 am to 8:18 pm.